Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.
Identifieur interne : 002F72 ( Main/Exploration ); précédent : 002F71; suivant : 002F73Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.
Auteurs : Alma Balestrazzi [Italie] ; Valentina Agoni ; Aldo Tava ; Pinarosa Avato ; Elisa Biazzi ; Elena Raimondi ; Anca Macovei ; Daniela CarboneraSource :
- Physiologia plantarum [ 1399-3054 ] ; 2011.
Descripteurs français
- KwdFr :
- Cellules cultivées (MeSH), Espèces réactives de l'oxygène (métabolisme), Extraits de plantes (pharmacologie), Medicago sativa (composition chimique), Monoxyde d'azote (biosynthèse), Mort cellulaire (effets des médicaments et des substances chimiques), Populus (effets des médicaments et des substances chimiques), Populus (métabolisme), Racines de plante (composition chimique), Saponines (isolement et purification), Saponines (pharmacologie), Survie cellulaire (MeSH).
- MESH :
- biosynthèse : Monoxyde d'azote.
- composition chimique : Medicago sativa, Racines de plante.
- effets des médicaments et des substances chimiques : Mort cellulaire, Populus.
- isolement et purification : Saponines.
- métabolisme : Espèces réactives de l'oxygène, Populus.
- pharmacologie : Extraits de plantes, Saponines.
- Cellules cultivées, Survie cellulaire.
English descriptors
- KwdEn :
- Cell Death (drug effects), Cell Survival (MeSH), Cells, Cultured (MeSH), Medicago sativa (chemistry), Nitric Oxide (biosynthesis), Plant Extracts (pharmacology), Plant Roots (chemistry), Populus (drug effects), Populus (metabolism), Reactive Oxygen Species (metabolism), Saponins (isolation & purification), Saponins (pharmacology).
- MESH :
- chemical , biosynthesis : Nitric Oxide.
- chemistry : Medicago sativa, Plant Roots.
- drug effects : Cell Death, Populus.
- chemical , isolation & purification : Saponins.
- metabolism : Populus, Reactive Oxygen Species.
- chemical , pharmacology : Plant Extracts, Saponins.
- Cell Survival, Cells, Cultured.
Abstract
The present work reports on the biological activity of alfalfa (Medicago sativa) saponins on white poplar (Populus alba, cultivar 'Villafranca') cell suspension cultures. The extracts from alfalfa roots, aerial parts and seeds were characterized for their saponin content by means of thin layer chromatography (TLC) and electrospray ionisation coupled to mass spectrometry. The quantitative saponin composition from the different plant extracts was determined considering the aglycone moieties and determined by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) analyses. Only soyasapogenin I was detected in the seed extract while several other saponins were found in the root and leaf extracts. Actively proliferating white poplar cell cultures were challenged with the different saponin extracts. Only alfalfa root saponins, at 50 µg ml⁻¹, induced significant cell death rates (75.00 ± 4.90%). Different cell subpopulations with peculiar cell death morphologies were observed and the programmed cell death (PCD)/necrosis ratio was reduced at increasing saponin concentrations. Enhancement of nitric oxide (NO) production was observed in white poplar cells treated with root saponins (RSs) at 50 µg ml⁻¹ and release of reactive oxygen species (ROS) in the culture medium was also demonstrated. Saponin-induced NO production was sensitive to sodium azide and N(G)-monomethyl-L-arginine, two specific inhibitors of distinct pathways for NO biosynthesis in plant cells.
DOI: 10.1111/j.1399-3054.2010.01436.x
PubMed: 21128946
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.</title><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Agoni, Valentina" sort="Agoni, Valentina" uniqKey="Agoni V" first="Valentina" last="Agoni">Valentina Agoni</name></author><author><name sortKey="Tava, Aldo" sort="Tava, Aldo" uniqKey="Tava A" first="Aldo" last="Tava">Aldo Tava</name></author><author><name sortKey="Avato, Pinarosa" sort="Avato, Pinarosa" uniqKey="Avato P" first="Pinarosa" last="Avato">Pinarosa Avato</name></author><author><name sortKey="Biazzi, Elisa" sort="Biazzi, Elisa" uniqKey="Biazzi E" first="Elisa" last="Biazzi">Elisa Biazzi</name></author><author><name sortKey="Raimondi, Elena" sort="Raimondi, Elena" uniqKey="Raimondi E" first="Elena" last="Raimondi">Elena Raimondi</name></author><author><name sortKey="Macovei, Anca" sort="Macovei, Anca" uniqKey="Macovei A" first="Anca" last="Macovei">Anca Macovei</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21128946</idno><idno type="pmid">21128946</idno><idno type="doi">10.1111/j.1399-3054.2010.01436.x</idno><idno type="wicri:Area/Main/Corpus">002F89</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002F89</idno><idno type="wicri:Area/Main/Curation">002F89</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002F89</idno><idno type="wicri:Area/Main/Exploration">002F89</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.</title><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Agoni, Valentina" sort="Agoni, Valentina" uniqKey="Agoni V" first="Valentina" last="Agoni">Valentina Agoni</name></author><author><name sortKey="Tava, Aldo" sort="Tava, Aldo" uniqKey="Tava A" first="Aldo" last="Tava">Aldo Tava</name></author><author><name sortKey="Avato, Pinarosa" sort="Avato, Pinarosa" uniqKey="Avato P" first="Pinarosa" last="Avato">Pinarosa Avato</name></author><author><name sortKey="Biazzi, Elisa" sort="Biazzi, Elisa" uniqKey="Biazzi E" first="Elisa" last="Biazzi">Elisa Biazzi</name></author><author><name sortKey="Raimondi, Elena" sort="Raimondi, Elena" uniqKey="Raimondi E" first="Elena" last="Raimondi">Elena Raimondi</name></author><author><name sortKey="Macovei, Anca" sort="Macovei, Anca" uniqKey="Macovei A" first="Anca" last="Macovei">Anca Macovei</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Death (drug effects)</term><term>Cell Survival (MeSH)</term><term>Cells, Cultured (MeSH)</term><term>Medicago sativa (chemistry)</term><term>Nitric Oxide (biosynthesis)</term><term>Plant Extracts (pharmacology)</term><term>Plant Roots (chemistry)</term><term>Populus (drug effects)</term><term>Populus (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Saponins (isolation & purification)</term><term>Saponins (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées (MeSH)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Extraits de plantes (pharmacologie)</term><term>Medicago sativa (composition chimique)</term><term>Monoxyde d'azote (biosynthèse)</term><term>Mort cellulaire (effets des médicaments et des substances chimiques)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (métabolisme)</term><term>Racines de plante (composition chimique)</term><term>Saponines (isolement et purification)</term><term>Saponines (pharmacologie)</term><term>Survie cellulaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nitric Oxide</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Monoxyde d'azote</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Medicago sativa</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Medicago sativa</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Death</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Mort cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Saponins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Saponines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Extraits de plantes</term><term>Saponines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Extracts</term><term>Saponins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Survival</term><term>Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Survie cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present work reports on the biological activity of alfalfa (Medicago sativa) saponins on white poplar (Populus alba, cultivar 'Villafranca') cell suspension cultures. The extracts from alfalfa roots, aerial parts and seeds were characterized for their saponin content by means of thin layer chromatography (TLC) and electrospray ionisation coupled to mass spectrometry. The quantitative saponin composition from the different plant extracts was determined considering the aglycone moieties and determined by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) analyses. Only soyasapogenin I was detected in the seed extract while several other saponins were found in the root and leaf extracts. Actively proliferating white poplar cell cultures were challenged with the different saponin extracts. Only alfalfa root saponins, at 50 µg ml⁻¹, induced significant cell death rates (75.00 ± 4.90%). Different cell subpopulations with peculiar cell death morphologies were observed and the programmed cell death (PCD)/necrosis ratio was reduced at increasing saponin concentrations. Enhancement of nitric oxide (NO) production was observed in white poplar cells treated with root saponins (RSs) at 50 µg ml⁻¹ and release of reactive oxygen species (ROS) in the culture medium was also demonstrated. Saponin-induced NO production was sensitive to sodium azide and N(G)-monomethyl-L-arginine, two specific inhibitors of distinct pathways for NO biosynthesis in plant cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21128946</PMID><DateCompleted><Year>2011</Year><Month>05</Month><Day>05</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>141</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.</ArticleTitle><Pagination><MedlinePgn>227-38</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1399-3054.2010.01436.x</ELocationID><Abstract><AbstractText>The present work reports on the biological activity of alfalfa (Medicago sativa) saponins on white poplar (Populus alba, cultivar 'Villafranca') cell suspension cultures. The extracts from alfalfa roots, aerial parts and seeds were characterized for their saponin content by means of thin layer chromatography (TLC) and electrospray ionisation coupled to mass spectrometry. The quantitative saponin composition from the different plant extracts was determined considering the aglycone moieties and determined by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) analyses. Only soyasapogenin I was detected in the seed extract while several other saponins were found in the root and leaf extracts. Actively proliferating white poplar cell cultures were challenged with the different saponin extracts. Only alfalfa root saponins, at 50 µg ml⁻¹, induced significant cell death rates (75.00 ± 4.90%). Different cell subpopulations with peculiar cell death morphologies were observed and the programmed cell death (PCD)/necrosis ratio was reduced at increasing saponin concentrations. Enhancement of nitric oxide (NO) production was observed in white poplar cells treated with root saponins (RSs) at 50 µg ml⁻¹ and release of reactive oxygen species (ROS) in the culture medium was also demonstrated. Saponin-induced NO production was sensitive to sodium azide and N(G)-monomethyl-L-arginine, two specific inhibitors of distinct pathways for NO biosynthesis in plant cells.</AbstractText><CopyrightInformation>Copyright © Physiologia Plantarum 2010.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balestrazzi</LastName><ForeName>Alma</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Agoni</LastName><ForeName>Valentina</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Tava</LastName><ForeName>Aldo</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Avato</LastName><ForeName>Pinarosa</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Biazzi</LastName><ForeName>Elisa</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Raimondi</LastName><ForeName>Elena</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Macovei</LastName><ForeName>Anca</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Carbonera</LastName><ForeName>Daniela</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>12</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012503">Saponins</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000455" MajorTopicYN="N">Medicago sativa</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012503" MajorTopicYN="N">Saponins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>12</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>12</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21128946</ArticleId><ArticleId IdType="doi">10.1111/j.1399-3054.2010.01436.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Agoni, Valentina" sort="Agoni, Valentina" uniqKey="Agoni V" first="Valentina" last="Agoni">Valentina Agoni</name><name sortKey="Avato, Pinarosa" sort="Avato, Pinarosa" uniqKey="Avato P" first="Pinarosa" last="Avato">Pinarosa Avato</name><name sortKey="Biazzi, Elisa" sort="Biazzi, Elisa" uniqKey="Biazzi E" first="Elisa" last="Biazzi">Elisa Biazzi</name><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name><name sortKey="Macovei, Anca" sort="Macovei, Anca" uniqKey="Macovei A" first="Anca" last="Macovei">Anca Macovei</name><name sortKey="Raimondi, Elena" sort="Raimondi, Elena" uniqKey="Raimondi E" first="Elena" last="Raimondi">Elena Raimondi</name><name sortKey="Tava, Aldo" sort="Tava, Aldo" uniqKey="Tava A" first="Aldo" last="Tava">Aldo Tava</name></noCountry><country name="Italie"><noRegion><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F72 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F72 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21128946
|texte= Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21128946" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |